Research Article

Peroxidase as the Major Protein Constituent in Areca Nut and Identification of Its Natural Substrates

Figure 4

Detection of the natural substrate(s) in the areca nut. (a) Wavelength scanning between 380 and 600 nm after mixing the AN extract with hydrogen peroxide, guaiacol, or water. (b) Measurement of absorbance increase at 440 nm for the AN extract in the presence of hydrogen peroxide. (c) LC/MS analysis of the AN extract in the presence of water (upper panel) or hydrogen peroxide (lower panel).