Inhibitory Effects of Hwangryunhaedok-Tang in 3T3-L1 Adipogenesis by Regulation of Raf/MEK1/ERK1/2 Pathway and PDK1/Akt Phosphorylation

<table>HRT inhibits the expression of transcription factors in differentiation of 3T3-L1 cells. Preadipocytes were induced to differentiate with extract of HRT (200 <i>μ</i>g/mL) and harvest at 2 h, day 4, and day 8 during the differentiation period. Panels are (a) the mRNA of C/EBP-<i>β</i>, (b) the mRNA of C/EBP-<i>α</i>, and (c) the mRNA of PPAR-<i>γ</i>. Panel (d) indicates the protein expression level of major factors. Values are mean ± S.D. of data from three separate experiments; each experiment was performed in triplicate. <svg height="13.95" id="M17" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 73.300003 13.95" width="73.300003" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> versus control. Treatment of PD98059 (50 <i>μ</i>M) and LY294002 (10 <i>μ</i>M) is positive control for HRT treatment. </table>

Evidence-Based Complementary and Alternative Medicine

fig2

Figure 2

Figure 2: Inhibitory Effects of Hwangryunhaedok-Tang in 3T3-L1 Adipogenesis by Regulation of Raf/MEK1/ERK1/2 Pathway and PDK1/Akt Phosphorylation 