Inhibitory Effects of Hwangryunhaedok-Tang in 3T3-L1 Adipogenesis by Regulation of Raf/MEK1/ERK1/2 Pathway and PDK1/Akt Phosphorylation

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td align="left">Gene name</td><td align="center">Forward primer</td><td align="center">Reverse primer</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left">PPAR-<svg height="12.175" id="M1" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 8.6374998 12.175" width="8.6374998" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,7.675)"><path d="M478 372q0 -39 -31 -97t-61 -98t-78 -98q-45 -55 -73 -102q-13 -79 -13 -197q-11 -11 -43.5 -25.5t-53.5 -15.5l-15 17q5 35 26 101.5t47 123.5q8 72 -1.5 174.5t-37.5 178.5q-14 37 -29 37q-20 0 -67 -65l-25 21q37 60 73 90.5t63 30.5q47 0 72 -112q13 -56 17.5 -141.5
t0.5 -143.5h2q155 193 155 297q0 26 -12 47q-5 8 -5 15q0 16 12.5 27t29.5 11q21 0 34 -21t13 -55z" id="x1D6FE"></path></g>
</svg></td><td align="center">TCACAAGAGGTGACCCAATG</td><td align="center">CCATCCTTCACAAGCATGAA</td></tr><tr><td align="left">C/EBP-<svg height="16.6625" id="M2" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 10 16.6625" width="10" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,12.162)"><path d="M558 587q0 -32 -14 -61t-40 -53.5t-48.5 -41t-54.5 -36.5q144 -51 144 -174q0 -55 -43.5 -108t-104.5 -87q-77 -42 -131 -42q-31 0 -54 20t-31 47l11 18q48 -29 108 -29q79 0 119.5 43t40.5 109t-44.5 107.5t-119.5 50.5l22 47q34 1 65 21q96 61 96 157q0 42 -24 67.5
t-62 25.5q-24 0 -43.5 -9t-35 -29.5t-27 -44t-22.5 -63t-19.5 -75.5t-18.5 -91q-57 -294 -68 -380q-26 -190 -35 -200q-26 -31 -97 -37l-4 26q19 9 48 170l77 413q23 121 52.5 187.5t83.5 114.5q70 62 148 62q51 0 88.5 -34t37.5 -91z" id="x1D6FD"></path></g>
</svg></td><td align="center">GTTTCGGGAGTTGATGCAATC</td><td align="center">AACAACCCCGCAGGAACAT</td></tr><tr><td align="left">C/EBP-<svg height="7.9499998" id="M3" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 9.7749996 7.9499998" width="9.7749996" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,7.675)"><path d="M545 106q-67 -118 -134 -118q-24 0 -40 37.5t-30 129.5h-2q-47 -72 -103 -119.5t-108 -47.5q-47 0 -76 45.5t-29 119.5q0 113 85 204t174 91q47 0 70 -33.5t43 -119.5h3q32 47 80 140l55 13l10 -9q-47 -80 -138 -201q17 -99 27.5 -136t22.5 -37q23 0 69 61zM333 204
q-14 98 -31 149.5t-50 51.5q-49 0 -94 -70t-45 -164q0 -55 15.5 -86t40.5 -31q70 0 164 150z" id="x1D6FC"></path></g>
</svg></td><td align="center">GTGTGCACGTCTATGCTAAACCA</td><td align="center">GCCGTTAGTGAAGAGTCTCAGTTTG</td></tr><tr><td align="left">aP2</td><td align="center">CCAATGAGCAAGTGGCAAGA</td><td align="center">GATGCCAGGCTCCAGGATAG</td></tr><tr><td align="left">LPL</td><td align="center">GGCCAGATTCATCAACTGGAT</td><td align="center">GCTCCAAGGCTGTACCCTAAG</td></tr><tr><td align="left">Adiponectin</td><td align="center">GGAGATGCAGGTCTTCTTGGT</td><td align="center">TCCTGATACTGGTCGTAGGTGAA</td></tr><tr><td align="left">SCD-1</td><td align="center">CCGGAGACCTTAGATCGA</td><td align="center">TAGCCTGTAAAAGATTTCTGCAAACC</td></tr><tr><td align="left">18s</td><td align="center">CATTCGAACGTCTGCCCTATC</td><td align="center">CCTGCTGCCTTCCTTGGA</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Evidence-Based Complementary and Alternative Medicine

tab1

Table 1

Table 1: Inhibitory Effects of Hwangryunhaedok-Tang in 3T3-L1 Adipogenesis by Regulation of Raf/MEK1/ERK1/2 Pathway and PDK1/Akt Phosphorylation 