Timosaponin AIII Suppresses Hepatocyte Growth Factor-Induced Invasive Activity through Sustained ERK Activation in Breast Cancer MDA-MB-231 Cells
Figure 3
Effects of timosaponin AIII on HGF-induced invasive activity in MDA-MB-231 cells. The migration assay (a) was used with a cell culture insert, and in vitro invasiveness of MDA-MB-231 cells was assessed using a modified Boyden chamber (b, c) as described in Section 2. TAIII concentration dependently (10−8~10−6 M) suppressed HGF-induced invasive activity, either by migration assay or by invasion assay. The data obtained from invasion assay was quantified (c). The arrow indicates the invaded cells on the membrane of Boyden chamber. * compared to serum free; # compared to HGF (15 ng/mL) alone-treat group (one-way ANOVA, followed by Dunnett’s test).
