Research Article

Antiproliferative Action of Conjugated Linoleic Acid on Human MCF-7 Breast Cancer Cells Mediated by Enhancement of Gap Junctional Intercellular Communication through Inactivation of NF-κB

Figure 2

Induction of apoptosis in MCF-7 cells treated with 40 μM c9,t11-CLA, t10,c12-CLA, and LA for 48 h. (a) Histograms of cells stained with propidium iodide and analyzed by flow cytometry. The peak area of M1, M2, M3, and M4 represents the cell population of Sub-G1, G0/G1, S, and G2/M phases, respectively. (b) Quantification of Sub-G1 cell population of (a), which is indicative of apoptosis. (c) Hoechst 33258 staining followed by fluorescence microscopic analysis of cells; arrows indicate fragmented or condensed nuclei. (d) Western blot analyses of the key executor enzyme of apoptotic cell death, activated caspase-3. The band intensities relative to control cells were quantified. Values are expressed as means ± standard deviations ( ). Means with different lowercase letters are significantly different at by Duncan’s multiple-range test.