5,7-Dihydroxyflavone Enhances the Apoptosis-Inducing Potential of TRAIL in Human Tumor Cells via Regulation of Apoptosis-Related Proteins

Figure 2

Interactive effects of 5,7-dihydroxyflavone and TRAIL on cell viability. (A), HepG2 cells were treated with various concentrations of 5,7-dihydroxyflavone and/or TRAIL. Cell viability was measured at the end of 72 h by MTT assay. Values marked with asterisk(s) are significantly different (^∗𝑃<0.05, ^∗∗𝑃<0.01) from control. Data are mean ± SD. (B), HepG2 cells were treated with 5,7-dihydroxyflavone and/or TRAIL for indicated lengths of time. Cell viability was measured by MTT assay. Values marked with asterisk(s) are significantly different (^∗𝑃<0.05, ^∗∗𝑃<0.01) from control. Data are mean ± SD. (C), light micrograph of HepG2 cells treated with 5,7-dihydroxyflavone and/or TRAIL for 24 h (×200). (a, control; b, 6 nM TRAIL; c, 20 μM 5,7-dihydroxyflavone; d, 60 nM TRAIL; e, 6 nM TRAIL + 20 μM 5,7-dihydroxyflavone). (D), Jurkat cells were treated with 5,7-dihydroxyflavone and/or TRAIL for 48 h and cell viability was measured by MTT assay. Values marked with asterisk(s) are significantly different (^∗𝑃<0.05, ^∗∗𝑃<0.01) from control. Data are mean ± SD. (E), Hela cells were treated with 5,7-dihydroxyflavone and/or TRAIL for 72 h and cell viability was measured by MTT assay. Values marked with asterisk(s) are significantly different (^∗𝑃<0.05, ^∗∗𝑃<0.01) from control. Data are mean ± SD. (F), L-O2 cells were treated with various concentrations of 5,7-dihydroxyflavone and/or TRAIL. Cell viability was measured by MTT assay. Data are mean ± SD.