4-Hydroxy-17-methylincisterol from Agaricus blazei Decreased Cytokine Production and Cell Proliferation in Human Peripheral Blood Mononuclear Cells via Inhibition of NF-AT and NF-𝜿B Activation

Figure 10

Effects of 4-HM on cyclins protein expression in PBMCs. (a) PBMC (1×10⁷ cells) were treated with medium, DMSO (0.1%), or 4-HM (5, 10 μM) in the presence or absence of PHA (5 μg/mL) for 72 hr. Lysates (50 μg/mL) were collected and run on a 10% SDS-PAGE gel and analyzed by immunoblotting with anti-cyclin D3, cyclin E, cyclin A or cyclin B. (b) The ratio of cyclin to 𝛽-tubulin was shown in bar graph. Each bar represents mean ± SD from three independent experiments. ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.001: versus DMSO+PHA control.

(a)

(b)