4-Hydroxy-17-methylincisterol from Agaricus blazei Decreased Cytokine Production and Cell Proliferation in Human Peripheral Blood Mononuclear Cells via Inhibition of NF-AT and NF-𝜿B Activation

Figure 6

Effects of 4-HM on NF-AT and NF-𝜅B activation. Jurkat cells (5×10⁴) stably transfected with pGL4.30 (luc2P/NFAT-RE/Hygro or luc2P/NF𝜅B-RE/Hygro) were cultured with PHA (5 μg/mL) in the presence or absence of CsA (2 μM), PDTC (20 μM), or 4-HM (2.5, 5, and 10 μM) for 4 hr. Total cell lysates were extracted with 1x reporter lysis buffer (Promega, USA), then 10 μg of total cell lysates were used to determine luciferase activity by the Luciferase Assay System (Promega, USA). Each bar is the mean±SD of three independent experiments. ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.001: versus PHA+DMSO control.

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