4-Hydroxy-17-methylincisterol from Agaricus blazei Decreased Cytokine Production and Cell Proliferation in Human Peripheral Blood Mononuclear Cells via Inhibition of NF-AT and NF-B Activation
Figure 7
Effects of 4-HM on Ca2+ mobilization in PBMC stimulated with PHA. (a) PBMC were loaded with 1 μM Fluo-3-AM at 37°C for 30 min. The cells were then resuspended in RPMI-1640 medium without phenol red to a concentration of cells/mL. In each experiment, 0.5 mL of equilibrated PBMC suspension was added with 2.5 μL of DMSO (0.1%) or 4-HM (2.5, 5, or 10 μM) at the 30th sec then stimulated with 2.5 μL of PHA (5 μg/mL) at the 110th sec and the changes in fluorescence with time were recorded. The fluorescent activity was recorded by an F-4500 fluorescence spectrophotometer (Hitachi, Tokyo, Japan) with multiwavelength time scan program. (b) The graph represents relative fluorescent units (% of DMSO control) of each test and each bar is the of six independent experiments. : versus DMSO control.
