Metabolomics Study of Resina Draconis on Myocardial Ischemia Rats Using Ultraperformance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry Combined with Pattern Recognition Methods and Metabolic Pathway Analysis
Table 1
Identified plasma metabolite markers of MI rats.
No
Retention time (min)
Exact mass
ID
Identified results
MI versus sham*
RD versus MI#
ID versus MIΔ
Pathway
1
1.23
182.0813
181.0739
C00082
L-tyrosine
↑
↓
Phenylalanine, tyrosine, and tryptophan biosynthesis
2
10.20
318.3007
317.2930
C12144
Phytosphingosine
↑
↓
Sphingolipid metabolism
3
11.38
302.3057
301.2981
C00836
Sphinganine
↑
↓
Sphingolipid metabolism
4
1.18
204.1233
203.1158
C02571
Acetylcarnitine
↑
↓
Fatty acid transportation
5
11.45
346.3317
345.0474
C00942
Cyclic GMP
↑
↓
Purine metabolism
6
12.64
330.3367
329.0525
C00575
Cyclic AMP
↑
↓
Purine metabolism
7
1.17
118.0866
117.0790
C00183
L-valine
↓
↑
Valine, leucine and isoleucine biosynthesis
8
12.76
520.3408
519.3325
C04230, HMDB10386
LPC(18:2)
↑
↓
Phospholipids metabolism
9
13.28
518.3227
517.3168
HMDB10388
LPC(18:3)
↑
↓
Phospholipids metabolism
10
18.99
121.0509
120.0436
HMDB01366
Purine
↑
↓
Purine metabolism
11
14.81
482.3243
481.3173
HMDB11130
LPE(18:0)
↓
↑
Phospholipids metabolism
12
17.91
780.5538
779.5465
C00157
PC(18:4)
↓
↑
Phospholipids metabolism
13
2.22
166.0866
165.0790
C00079
L-phenylalanine
↓
↑
Phenylalanine, tyrosine, and tryptophan biosynthesis
14
4.16
205.0977
204.0965
C00078
L-tryptophan
↓
↑
Phenylalanine, tyrosine, and tryptophan biosynthesis
15
13.27
496.3415
495.3325
C04230
LPC(16:0)
↑
↓
↓
Phospholipids metabolism
16
14.81
504.3063
503.3012
HMDB11516
LPE(20:3)
↓
↑
↑
Phospholipids metabolism
17
1.35
203.053
180.0634
NA
C6H12O6
↓
↑
↑
Unknown
18
10.28
274.2747
273.2668
C13915
Hexadecasphinganine
↑
↑
↑
Sphingolipid metabolism
19
12.77
542.3228
541.3168
C04230, HMDB10397
LPC(20:5)
↑
↑
↑
Phospholipids metabolism
20
1.18
150.0584
149.0511
C00073
Methionine
↑
↑
↑
Aminoacyl-tRNA biosynthesis
↑ or ↓ represents the up- or downregulation of the metabolites in MI group compared with the sham group; ↑ or ↓ represents the significant up- or downregulation of the metabolites in resina-draconis (RD-) treated group compared with the MI group; ↑ or ↓ represents the significant up- or down-regulation of the metabolites in isosorbide dinitrate- (ID, positive control drug) treated group compared with the MI group.
