Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 2

Research Article

Mechanisms Underlying the Antiproliferative and Prodifferentiative Effects of Psoralen on Adult Neural Stem Cells via DNA Microarray

Figure 2

Effects of Psoralen on the proliferation of NSC. Single NSCs were plated at a density of 5000 cells per well in PDL-coated 96-well plate for 12 h. Then, cells were subjected to 10 nM EdU for 2 h, followed by addition of 100 nM OA (b) or not (a). Then Edu immunofluorescence analysis was performed. The cell nuclei were counterstained with DAPI. The percentage of EdU-positive cells in total of 1000 cells was calculated. As a result, Psoralen significantly inhibited the DNA incorporation (c). Scale bars: 100 μm. Results were expressed as mean ± S.D. from four independent experiments. * versus control.
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(a)
452948.fig.002b
(b)
452948.fig.002c
(c)  % of EDU+ cells