Hyperglycemia Induces Altered Expressions of Angiogenesis Associated Molecules in the Trophoblast
Figure 2
The expression of cell-associated perlecan in trophoblasts upon treatment with high-glucose condition. To characterize perlecan expression profile upon treatment with high-glucose condition, time course analysis of perlecan immunoprecipitation was conducted in 3A-Sub-E cell lysates with pretreatment of 30 mM D-glucose for 24, 48, and 72 h. (a) Perlecan expression was electrophoresis and shown on a 6% SDS polyacrylamide gel stained with alcian blue and Coomassie blue G25 solutions. (b) Quantitative results of perlecan protein from cell lysates under different stimulations. The expression of perlecan was performed as mean SD by quantification of the relative intensity of the scanned smear bands from double stains with Coomassie blue and alcian blue (). The total intensity (30 mM Glu. + 5.6 mM Glu. + 30 mM Man.) from each time point was considered as 100. **. n.s., not significant.
