Hyperglycemia Induces Altered Expressions of Angiogenesis Associated Molecules in the Trophoblast
Figure 6
The effect of hyperglycemia on the expression of MMP inhibitors in 3A-Sub-E trophoblast cells. 3A-Sub-E cells were exposed to 30 mM D-glucose or to 5.6 mM of D-glucose (control) for 48 h. Cell lysates (250–370 μg) and the cultured conditioned media were collected for the antibody array analysis, respectively. (a) Representative dot image of the indicated MMP inhibitor. (b) Average intensity for each pair of cytokine spots was quantitatively measured by Scion Image software. Data are presented as mean SD (). (c) A gelatin zymography gel image for MMP2 and MMP 9 activity. *. **. Each cytokine is represented by duplicate spots as indicated, TIMP-1 (tissue inhibitors of metalloproteinase-1), TIMP-2 (tissue inhibitors of metalloproteinase-2), and control (HRP-conjugated antibody as a positive control).
