A Type II Arabinogalactan from Anoectochilus formosanus for G-CSF Production in Macrophages and Leukopenia Improvement in CT26-Bearing Mice Treated with 5-Fluorouracil
Figure 4
Effects of inhibitors on G-CSF mRNA expression and production in RAW 264.7 cells stimulated with AGAF (100 μg/mL). (a) G-CSF mRNA expression in RAW 264.7 cells that were pretreated with specific inhibitors or a medium for 60 min and then treated with 100 μg/mL of AGAF for 6 h. The G-CSF mRNA expression was examined via RT-PCR. (b) G-CSF production in RAW 264.7 cells that were pretreated with specific inhibitors or a medium for 60 min and then treated with 100 μg/mL of AGAF for 16 h. The levels of G-CSF were determined by ELISA. The values are presented as the mean SD; . **, and *** as compared to control group as analyzed by Dunnett’s test.
