In Vitro Screening for Antihepatic Steatosis Active Components within Coptidis Rhizoma Alkaloids Extract Using Liver Cell Extraction with HPLC Analysis and a Free Fatty Acid-Induced Hepatic Steatosis HepG2 Cell Assay

Table 1

Intracellular TG content of hepatic steatosis cell model treated with alkaloids.

Sample group	Magnoflorine	Columbamine	Jateorhizine	Epiberberine	Coptisine	Palmatine	Berberine
Control group	37±03	42±12	57±06	79±10	39±07	70±14	47±13
Model group	464±34^##	456±32^##	510±15^##	520±31^##	485±29^##	520±11^##	473±12^##
0.01 𝜇g/mL					424±15
0.04 𝜇g/mL					327±12^∗
0.2 𝜇g/mL	397±22	438±06	475±07	516±09	248±09^∗∗	528±15	390±04^∗
1.0 𝜇g/mL	350±19^∗	376±23^∗	475±01	468±29	240±03^∗∗	460±06^∗	308±08^∗∗
5.0 𝜇g/mL	336±06^∗	316±13^∗∗	509±19	390±13^∗	240±15^∗∗	410±27^∗∗	225±12^∗∗
25.0 𝜇g/mL	276±25^∗∗	234±21^∗∗	541±14	336±11^∗∗	231±06^∗∗	339±24^∗∗	183±03^∗∗

^*Indicates a significant difference compared with model group cells, and # indicates a significant difference compared with control group cells. Values were mean ± SD (𝑛=6) and expressed in 𝜇mol/g protein.
* 𝑃<0.05, **𝑃<0.01, ^##𝑃<0.01.