Aqueous Extract of Paeonia lactiflora and Paeoniflorin as Aggregation Reducers Targeting Chaperones in Cell Models of Spinocerebellar Ataxia 3
Flp-In 293 cells with ATXN3/Q14~75-GFP expression in an inducible fashion. (a) Western blot analysis of ATXN3/Q14~75-GFP protein expression using GFP antibody after two days of induction (+Dox). (b) Real-time PCR quantification of ATXN3/Q14~75-GFP RNA expression relatively to HPRT after two days of induction (+Dox). (c) Fluorescence microscopy images of ATXN3/Q14~75-GFP expression after six days of induction. The scale bar = 100 µm.