Aqueous Extract of Paeonia lactiflora and Paeoniflorin as Aggregation Reducers Targeting Chaperones in Cell Models of Spinocerebellar Ataxia 3
Figure 5
Enhancement of HSF1 and chaperone expression by paeoniflorin and the aqueous extract of P. lactiflora in ATXN3/Q75-GFP 293 cells. Cells were pre-treated with paeoniflorin (100 nM) or herb (10 μg/mL) for 8 hours and ATXN3/Q75-GFP expression induced for 6 days. Relative HSF1, HSPA8, and HSPA1A expressions were analyzed by western blot analysis using β-actin as a loading control.
