Research Article

Aqueous Extract of Paeonia lactiflora and Paeoniflorin as Aggregation Reducers Targeting Chaperones in Cell Models of Spinocerebellar Ataxia 3

Figure 7

Reduction of aggregation by paeoniflorin and the aqueous extract of P. lactiflora in ATXN3/Q75-GFP SH-SY5Y cells. (a) Western blot analysis using GFP and GAPDH antibodies after two days induction (+Dox). (b) Microscopic images of differentiated SH-SY5Y cells expressing ATXN3/Q14/75-GFP for 6 days (the scale bar = 20 μm). (c) Relative aggregation after treatment with paeoniflorin (100 nM) or the aqueous extract of P. lactiflora (10 μg/mL) for 6 days. To normalize, the relative aggregation level in untreated cells is set as 100%.