In Vitro Global Gene Expression Analyses Support the Ethnopharmacological Use of Achyranthes aspera

Table 2

(a) List of genes whose expression was downregulated at all the three time points. (b) List of genes whose expression was up regulated at all the three time points. Average fold change in gene expression and the corresponding SD for the three different time points is given. Genes that showed FC of ±2 and FDR of <0.01 were selected.

(a)

No.	Gene name	NCBI description	AVE FC	SD
1	SYTL5	Synaptotagmin-like 5	−2.92	1.88
2	KGFLP1	Fibroblast growth factor 7 pseudogene	−2.80	1.42
3	ARPC3P5	Actin related protein 2/3 complex, subunit 3 pseudogene 5	−2.63	1.88
4	OSBPL8	Oxysterol binding protein-like 8	−2.62	0.01
5	C11orf10	Chromosome 11 open reading frame 10	−2.56	1.09
6	DHX9	DEAH (Asp-Glu-Ala-His) box polypeptide 9	−2.51	0.77
7	C1orf53	Chromosome 1 open reading frame 53	−2.45	1.16
8	VWF	Von Willebrand factor	−2.40	0.59
9	AF038194	Homo sapiens clone 23821 mRNA sequence	−2.35	0.51
10	POM121	POM121 transmembrane nucleoporin	−2.33	0.36
11	THC2671048	Q3DWD9_CHLAU (Q3DWD9) YLP motif, partial (6%)	−2.33	0.71
12	TOM1L2	Target of myb1-like 2 (chicken)	−2.33	0.76
13	CAPZA3	Capping protein (actin filament) muscle Z-line, alpha 3	−2.33	0.82
14	CYSLTR2	Cysteinyl leukotriene receptor 2	−2.30	0.54
15	ANKRD33B	Ankyrin repeat domain 33B	−2.27	0.61
16	AA889371	am40h08.s1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE:1471263 3′ similar to SW:COQ1_YEAST P18900 HEXAPRENYL PYROPHOSPHATE SYNTHETASE	−2.26	0.67
17	FAM101A	Family with sequence similarity 101, member A	−2.26	0.91
18	IRF5	Interferon regulatory factor 5	−2.25	0.68
19	EGFLAM	EGF-like, fibronectin type III, and laminin G domains	−2.25	0.72
20	SIRT3	NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform a	−2.20	0.65
21	NEK8	NIMA (never in mitosis gene a)—related kinase 8	−2.19	1.07
22	ROD1	Polypyrimidine tract binding protein 3	−2.18	0.60
23	RBM26	RNA binding motif protein 26	−2.18	1.05
24	HYAL1	Hyaluronoglucosaminidase 1 (HYAL1), transcript variant 1, noncoding RNA	−2.12	0.45
25	IL2	Interleukin 2	−2.10	0.17
26	LRRC20	Leucine-rich repeat-containing protein 20 isoform 3	−2.10	0.67
27	THC2635921		−2.09	0.90
28	GJD4	Gap junction protein, delta 4, 40.1 kDa	−2.09	0.52
29	SNAPC1	Small nuclear RNA activating complex, polypeptide 1, 43 kDa	−2.06	0.50
30	GNG13	Guanine nucleotide binding protein (G protein), gamma 13	−2.00	0.45
31	POLR2A	Polymerase (RNA) II (DNA directed) polypeptide A, 220 kDa	−1.99	0.23
32	CD44	CD44 molecule (Indian blood group)	−1.97	0.87
33	CR742006	CR742006 Soares_testis_NHT Homo sapiens cDNA clone IMAGp971J1256; IMAGE:1048737 5′, mRNA sequence	−1.96	0.53
34	THC2709441	Q4VIX2_DROBU (Q4VIX2) Dbuz∖abd-A-PB, partial (4%)	−1.95	0.77
35	PIGU	Phosphatidylinositol glycan anchor biosynthesis, class U	−1.95	0.25
36	KRT14	Keratin 14	−1.91	0.62
37	BE564275	601343077F1 NIH_MGC_53 Homo sapiens cDNA clone IMAGE:3685338 5′, mRNA sequence	−1.90	0.59
38	ZNF622	Zinc finger protein 622	−1.89	0.76
39	BQ060012	AGENCOURT_6793913 NIH_MGC_99 Homo sapiens cDNA clone IMAGE:5816175 5′, mRNA sequence	−1.88	0.28
40	LCE1D	Late cornified envelope 1D	−1.88	0.13
41	BC034623	Homo sapiens cDNA clone IMAGE:4837603	−1.86	0.45
42	SLA	Src-like-adaptor	−1.85	0.30
43	HLA-DOB	Major histocompatibility complex, class II, DO beta	−1.85	0.57
44	FOXL1	Forkhead box L1	−1.84	0.34
45	B3GNT9	UDP-GlcNAc:betaGal beta-1, 3-N-acetylglucosaminyltransferase 9	−1.84	0.66
46	SLC15A1	Solute carrier family 15 (oligopeptide transporter), member 1	−1.82	0.43
47	HADHB	hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit	−1.79	0.61
48	HSPA14	Heat shock 70 kDa protein 14	−1.78	0.43
49	BC092421	Homo sapiens cDNA clone IMAGE:30378758	−1.76	0.39
50	POC1A	POC1 centriolar protein homolog A (Chlamydomonas)	−1.75	0.27
51	ACVR1	Activin A receptor, type I	−1.72	0.41
52	TPSD1	Tryptase delta 1	−1.68	0.33
53	MAPK8IP3	Mitogen-activated protein kinase 8 interacting protein 3	−1.67	0.40
54	UCHL5	Ubiquitin carboxyl-terminal hydrolase L5	−1.61	0.41

(b)

No.	Gene name	NCBI description	AVE FC	SD
1	MBD6	Methyl-CpG binding domain protein 6	1.58	0.44
2	BG536553	602564961F1 NIH_MGC_77 Homo sapiens cDNA clone IMAGE:4689518 5′, mRNA sequence	1.64	0.34
3	APOL1	Apolipoprotein L, 1	1.64	0.44
4	LOC728537	Uncharacterized LOC728537	1.69	0.52
5	THC2624002	Q9BXR7_HUMAN (Q9BXR7) Interleukin 10 (Fragment), partial (93%)	1.72	0.49
6	CASKIN2	CASK interacting protein 2	1.77	0.41
7	PML	Promyelocytic leukemia	1.78	0.66
8	ZC3HAV1L	Zinc finger CCCH-type, antiviral 1-like	1.81	0.25
9	THC2653001	BX098637 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGp998F16386; IMAGE:200847, mRNA sequence	1.81	0.67
10	LOC728344	Glutaredoxin 3 pseudogene	1.88	0.56
11	METRNL	Meteorin, glial cell differentiation regulator-like	1.89	0.89
12	C8orf75	Long intergenic nonprotein coding RNA 589	1.91	0.81
13	AK021715	Homo sapiens cDNA FLJ11653 fis, clone HEMBA1004538	1.91	0.86
14	TMCO1	Transmembrane and coiled-coil domains 1	1.99	0.36
15	UQCC	Ubiquinol-cytochrome c reductase complex chaperone	2.00	0.41
16	RPL7P48	Ribosomal protein L7 pseudogene 48	2.14	0.73
17	AF483645	Homo sapiens capacitative calcium channel protein Trp1 mRNA, partial cds; alternatively spliced	2.17	0.67
18	TAF9B	TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31 kDa	2.31	0.37
19	AK026477	Homo sapiens cDNA:FLJ22824 fis, clone KAIA3991	2.34	0.39
20	BBS12	Bardet-Biedl syndrome 12	2.37	0.48
21	THC2624048	Q964F8_PLAFA (Q964F8) Merozoite surface protein 8, partial (3%)	2.37	0.44
22	TBP	TATA box binding protein	2.37	0.50
23	BC034627	Homo sapiens cDNA clone IMAGE:4839213	2.38	0.36
24	JAG2	Jagged 2	2.42	0.49
25	TMEM8	Transmembrane protein 8A	2.42	0.43
26	CARD18	Caspase recruitment domain family, member 18	2.51	0.59
27	AA020958	ze65a02.s1 Soares retina N2b4HR Homo sapiens cDNA clone IMAGE:363818 3′, mRNA sequence	2.52	0.48
28	ICAM1	Intercellular adhesion molecule 1	2.63	0.77