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Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 475386, 10 pages
http://dx.doi.org/10.1155/2013/475386
Research Article

Rubi Fructus (Rubus coreanus) Inhibits Differentiation to Adipocytes in 3T3-L1 Cells

1Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Jeonbuk 570-749, Republic of Korea
2College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea
3Department of Pharmacology, College of Oriental Medicine, Sangji University, Wonju-si, Gangwon-do 220-702, Republic of Korea
4Department of Cosmeceutical Science, Daegu Hanny University, Yugok-dong, Kyungsan 712-715, Republic of Korea
5Center for Metabolic Function Regulation, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea

Received 2 April 2013; Revised 14 August 2013; Accepted 21 August 2013

Academic Editor: Seong-Gyu Ko

Copyright © 2013 Mi-Young Jeong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Rubi Fructus (RF) is known to exert several pharmacological effects including antitumor, antioxidant, and anti-inflammatory activities. However, its antiobesity effect has not been reported yet. This study was focused on the antidifferentiation effect of RF extract on 3T3-L1 preadipocytes. When 3T3-L1 preadipocytes were differentiating into adipocytes, 10–100 μg/mL of RF was added. Next, the lipid contents were quantified by Oil Red O staining. RF significantly reduced lipid accumulation and downregulated the expression of peroxisome proliferator-activated receptor γ (PPARγ), CCAAT0-enhancer-binding proteins α (C/EBPα), adipocyte fatty acid-binding protein 2 (aP2), resistin, and adiponectin in ways that were concentration dependent. Moreover, RF markedly upregulated liver kinase B1 and AMP-activated protein kinase (AMPK). Interestingly, pretreatment with AMPKα siRNA and RF downregulated the expression of PPARγ and C/EBPα protein as well as the adipocyte differentiation. Our study shows that RF is capable of inhibiting the differentiation of 3T3-L1 adipocytes through the modulation of PPARγ, C/EBPα, and AMPK, suggesting that it has a potential for therapeutic application in the treatment or prevention of obesity.