Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 5

Research Article

Rubi Fructus (Rubus coreanus) Inhibits Differentiation to Adipocytes in 3T3-L1 Cells

Figure 5

Effects of RF on the phosphorylation of AMPK during 3T3-L1 differentiation. 3T3-L1 preadipocytes were differentiated in the presence of RF (0, 10, 50, and 100 μg/mL) for 6 days. (a) p-LKB1, p-AMPK, and ACC protein levels were analyzed by Western blot analysis. (b) Densitometry analyses were presented as relative ratios of p-LKB1/GAPDH, p-AMPK/AMPK, and ACC/GAPDH. (c) 3T3-L1 cells were treated with RF (Day 4) in AMPKα siRNA pretreatment (Day 0). Cells were stained with Oil Red O at 6 days, and lipid contents were quantified. These experiments were conducted as independent experiments in triplicate. Data represent the mean ± S.D. where was considered a statistically significant difference from the differentiated control. (d) After AMPKα siRNA pretreatment and RF-treated differentiation, the protein expressions of AMPKα, PPARγ, and C/EBPα were determined by Western blot analysis.

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