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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 483286, 10 pages
Research Article

Chemical and Biological Assessment of Angelica Roots from Different Cultivated Regions in a Chinese Herbal Decoction Danggui Buxue Tang

1Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Kowloon, Hong Kong
2Department of Biology, Hanshan Normal University, Chaozhou, Guangdong 521041, China

Received 19 September 2012; Accepted 26 December 2012

Academic Editor: Mee-Ra Rhyu

Copyright © 2013 Wendy L. Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Table 1: Mass spectra properties of marker chemicals. For the MS/MS analysis, the suitable precursor ion and two product ions were chosen for acquisition in MRM mode for ferulic acid, butylphthalide, Z-butylidenephthalide, senkyunolide A, Z-ligustilide, and paeonol (internal standard). The fragmentor voltage and collision energy values were optimized to obtain the highest abundance. Agilent MassHunter so/ware was used for data acquisition and processing.

Supplementary Table 2: Calibration curves, LOD and LOQ of five markers. For the linearity, the calibration curve of each chemical was constructed using a range of concentrations of working standards, and each line was based on six different concentrations. The limit of detection (LOD) and limit of quantication (LOQ) were used to evaluate the sensitivity. The LOD was estimated with a signal 3 times higher than that of the baseline noise, while the LOQ was 10 times higher.

Supplementary Table 3: Precision, repeatability and recovery of markers. The precision was determined by intraday and interday variations, which were performed by analyzing standard solutions during a single day (n = 6) and on three executive days (n = 6), respectively. For repeatability test, five independent sample solutions were prepared in the procedures of sample preparation. The accuracy was evaluated as the percentage recovery of analytes in the spiked samples. The recoveries were calculated by the following formula: recovery (%) = 100 × (amount found – original amount)/amount spiked. RSD was used to describe precision, repeatability, and recovery.

  1. Supplementary Tables