Metabonomics Combined with UPLC-MS Chemical Profile for Discovery of Antidepressant Ingredients of a Traditional Chinese Medicines Formula, Chaihu-Shu-Gan-San

Figure 2

MVA of urine samples from all groups detected by NMR and UPLC-Q-TOF/MS. A1, B1, and C1: the spectra and chromatograms of urine samples. Black color stands for control group, red color for CUMS group, blue color for CSGS treated group, and green color for QZ treated group; A2, B2, and C2. The score plots of control, CUMS, CSGS, and QZ groups; A3, B3, and C3. OPLS-DA scores plots of CUMS versus model groups, CUMS versus CSGS groups, CUMS versus QZ groups (A3: 𝑄2𝑋_CUM=0.879, 𝑅²𝑌_CUM=0.998, 𝑄²_(CUM)=0.607, B3: 𝑄²𝑋_(CUM)=0.604, 𝑅²𝑌_CUM=0.994, 𝑄²_(CUM)=0.99; C3: 𝑄²𝑋_(CUM)=0.659, 𝑅²𝑌_CUM=0.999, 𝑄²_(CUM)=0.999); A4, B4, and C4: line plots, S-plots, and VIP-value plots of urine samples detected by NMR and UPLC-Q-TOF/MS, respectively.