In Vitro and In Vivo Anticancer Effects of Sterol Fraction from Red Algae Porphyra dentata
Figure 2
HPLC-ELSD chromatograms of Porphyra dentata F2 fraction (a) and a mixture of standards (cholesterol, β-sitosterol, and campesterol) (b). The retention time () of each major peak (Peak 1, Peak 2, and Peak 3) present in F2 fraction and each standard is shown in parentheses and on a time scale. Conditions for HPLC-ELSD: column, Mightysil RP-18 (H) column (250 × 4.5 mm); column temperature, 40°C; mobile phase, 95% methanol in water; flow rate: 1 mL/min; ELSD detector nebulizer temperature, 80°C.
