Evidence for the Primo Vascular System above the Epicardia of Rat Hearts
Figure 4
Comparative positions of a PN and a PV stained with (a) hematoxylin and eosin (H&E) and with (b) toluidine blue (TB). Image (a) shows a randomly cut PV (dotted circles) and a cross-sectioned PN (arrow) above the epicardium (EC). H&E staining of the PV and the PN suggest they are basophilic; however, the epicardium is eosinophilic. The inset image is a magnified view of a PN with sinuses (S). Image (b) shows a toluidine-blue-stained PN in which four mast cells (MC) are found. Among the MCs, one MC is just releasing from the PN. The scale bars of (a), (a)’s inset, and (b) are 30 μm, 12 μm, and 25 μm, respectively.
