Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 1

Research Article

Reishi Protein LZ-8 Induces FOXP3+ Treg Expansion via a CD45-Dependent Signaling Pathway and Alleviates Acute Intestinal Inflammation in Mice

Figure 1

LZ-8 stimulates Treg expansion in murine and human CD4+ T cells. Primary murine and human CD4+ T cells were cultured for 72 h, either in the absence of stimulation as the control (CTR), in the presence of stimulation with recombinant LZ-8 antibody (rLZ-8; 1 μg/mL), or in the presence of stimulation with anti-CD3/28 antibodies (CD3/CD28; 1 μg/mL each, (a, b). The cells were then harvested for flow cytometry analysis of CD25, FOXP3, and CTLA-4 expression. (c and d) The levels of IL-2, IL-10, IFN-γ, and TGF-β in the culture supernatants were measured by ELISA. (e) The cell proliferation of CD4+ T cells treated with or without PHA (2 μg/mL) and then cocultured with indicated CD4+ T cells was monitored by cell-trace CFSE fluorescence via flow cytometry.

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