NBM-T-L-BMX-OS01, Semisynthesized from Osthole, Is a Novel Inhibitor of Histone Deacetylase and Enhances Learning and Memory in Rats
Effects of SAHA and BMX on the novel object recognition test in rats. Control, SAHA-, and BMX-injected rats were subjected to the novel object recognition test. The retention time(s) spent in exploring the left object, right object, and novel object before (pre-) and after (post-) the right object was replaced with the novel object was recorded (a). After the experiment, the hippocampus was dissected for AcH4K12 western blotting. A representative gel pattern and quantitative analysis of AcH4K12 and the H4 level from the rat hippocampus (b). The HDAC8 activity in the hippocampus was analyzed (d). The data are the mean ± SEM. *, in the comparison of the control group; #, in the comparison between groups. ; . A two-way ANOVA followed by Newman-Keuls comparisons was applied for (a). A one-way ANOVA followed by Newman-Keuls comparisons was applied for (b) and (c).