The Antitumor Effects of Triterpenoid Saponins from the Anemone flaccida and the Underlying Mechanism

Figure 2

Effects of TS and triterpenoid saponins 1–5 on apoptosis rate of cancer cells. BEL-7402 ((a), (d)) and HepG2 cells ((b), (d)), as well as high COX-2 expressed HeLa cells induced by LPS treatment ((c), (d)), were then treated with 40.0 μg·mL⁻¹ TS or 40.0 μmol·L⁻¹ single triterpenoid saponins 1–5, respectively, for 24 h, and the apoptotic rate was determined by Annexin V-PI staining and flow cytometry for quantification. *𝑃<0.05, **𝑃<0.01 versus control (mean ± SD).

(a)  BEL-7402

(b) HepG2

(c) HeLa+LPS

(d)