In Vitro Antiproliferative Effect of Arthrocnemum indicum Extracts on Caco-2 Cancer Cells through Cell Cycle Control and Related Phenol LC-TOF-MS Identification
Figure 1
(a) Measurements of cell proliferation using MTT assay in human carcinoma Caco-2 cell line treated with A. indicum shoot extracts. Cells at 2 × 104 cells/mL were left untreated or were treated with 0.01, 0.1, 1, 10, 50, or 100 μg/mL of A. indicum for 72 h. Values represent the results of three independent experiments. (b) DAPI staining of Caco-2 cells treated with A. indicum. Cells at 2 × 104 cells/mL were incubated with 100 μg/mL of A. indicum for 72 h. Control cells were incubated with 0.4% DMSO. After 72 h, the nuclear morphologies of cells were examined using a fluorescent DNA-binding agent, DAPI. The DNA was analyzed using fluorescence microscopy. The arrow indicates mitotic cells with chromatin distribution. Results shown (a) and (b) are typical of 3 independent experiments.
