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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 536560, 5 pages
Research Article

A Method for the Observation of the Primo Vascular System in the Thoracic Duct of a Rat

1Department of Acupuncture & Moxibustion, Wonkwang University, Gwangju Medical Hospital, Gwangju 503-310, Republic of Korea
2Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, Republic of Korea
3Graduate School of Convergence Science Technology, Seoul National University, Suwon 443-270, Republic of Korea

Received 7 February 2013; Accepted 30 May 2013

Academic Editor: Walter J. Akers

Copyright © 2013 Sungha Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Even though the primo vascular system (PVS) has been observed in large caliber lymph vessels by several independent teams, the presence of the PVS in the thoracic duct has been reported by only one team, probably because reproducing the experiment is technically difficult. This brief report presents a new, relatively straightforward method, which is a simple modification of the previous method of dye injection into the lumbar node, to observe the PVS in a thoracic duct of a rat by injecting Alcian blue into the renal node. When this new method was applied to a rat, the branching of the primo vessel in the thoracic duct was clearly displayed. Thus, this new method is expected to extend the network of the PVS from abdominal lymph ducts to thoracic ones.