Curcumin Suppresses Metastasis via Sp-1, FAK Inhibition, and E-Cadherin Upregulation in Colorectal Cancer
Figure 5
Sp-1 transcriptional regulation via curcumin treatment. (a) Gene expression fold changes of five Sp-1 downstream genes, containing ADAM10, calmodulin, EPHB2, HDAC4, and SEPP1, were determined using qRT-PCR. Five genes transcriptionally activated by Sp-1 showed uniformly suppression via curcumin treatment. (b) The gene expression level of Sp-1 was measured by qRT-PCR. (c) Luciferase reporter assay quantifying the Sp-1 transcriptional activity changes in curcumin treated cells. HEK293T cells transiently transfected with Sp-1 reporter plasmid and Renilla control plasmid were treated with different concentrations of curcumin. The firefly and Renilla luciferase activities of the cell extracts were quantified using the Dual-Glo luciferase assay system. Data are presented as the ratio of firefly:Renilla luciferase activity. *.
