Curcumin Suppresses Metastasis via Sp-1, FAK Inhibition, and E-Cadherin Upregulation in Colorectal Cancer
Figure 6
The effect of curcumin on FAK expression and cell adhesion. (a) FAK mRNA levels were suppressed under curcumin treatment and detected by qRT-PCR. (b) Curcumin inhibits the protein expression and phosphorylation of FAK. HCT-116 cells were treated with different concentrations of curcumin for 24 h. The cell lysates were harvested and analyzed by specific antibodies against FAK and different tyrosine phosphorylation sites of FAK, including 397, 407, 576, 577, and 861. (c) Curcumin enhances cell adhesion ability. After pretreatment with serial curcumin for 24 h, cells were seeded on 96-well plates coated with different ECM, including collagen I, collagen III, collagen IV, collagen IX, laminin, and BSA as negative control. The noncoating control at the first lane was performed to control added cell number. Cells were allowed to adhere for 1 h at 37°C and then washed twice with PBS. Bound cells were stained by DAPI and analyzed by high content system. Data are presented as mean SD (). *.
