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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 545201, 9 pages
http://dx.doi.org/10.1155/2013/545201
Research Article

Microwave Saturation of Complex EPR Spectra and Free Radicals of Burnt Skin Treated with Apitherapeutic Agent

1Department of Community Pharmacy, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland
2Department of Biophysics, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland
3Institute of Computer Science, University of Silesia, 41-200 Sosnowiec, Poland
4Department of Clinical Chemistry and Laboratory Diagnostics, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland
5Center of Experimental Medicine, Medical University of Silesia in Katowice, 40-752 Katowice, Poland

Received 5 April 2013; Accepted 1 May 2013

Academic Editor: Ewelina Szliszka

Copyright © 2013 Pawel Olczyk et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The effect of microwave power on the complex electron paramagnetic resonance spectra of the burn matrix after the therapy with propolis was examined. The spectra were measured with microwaves in the range of 2.2–79 mW. Three groups of free radicals were found in the damaged skin samples. Their spectral lines evolve differently with the microwave power. In order to detect these free radical groups, the lineshape of the spectra was numerically analysed. The spectra were a superposition of three component lines. The best fit was obtained for the deconvolution of the experimental spectra into one Gauss and two Lorentz lines. The microwave power changes also the lineshape of the spectra of thermally injured skin treated with the conventional agent—silver sulphadiazine. The spectral changes were different for propolis and for silver sulphadiazine. The number of individual groups of free radicals in the wound bed after implementation of these two substances is not equal. It may be explained by a higher activity of propolis than of silver sulphadiazine as therapeutic agents.