Okadaic Acid, a Bioactive Fatty Acid from Halichondria okadai, Stimulates Lipolysis in Rat Adipocytes: The Pivotal Role of Perilipin Translocation
Figure 3
Immunofluorescent labeling of perilipin A/B in isolated intracellular lipid droplets. Adipocytes were incubated with buffer A, DMSO, isoproterenol (10 μM), or okadaic acid (1 μM) for 2 h at 37°C. Perilipins were assembled as a bright ring surrounding the surface of lipid droplets in buffer A control (a and b) and DMSO (c and d), but labeling for perilipins was weak on lipid droplets in the isoproterenol-stimulated group (e and f) and absent on lipid droplets in the okadaic-acid-stimulated group (g and h). Figures (a), (c), (e), and (g) show phase-contrast images of figures (b), (d), (f), and (h), respectively. Arrows indicate isolated intracellular lipid droplets. Bar = 20 μm.
