Resveratrol Downregulates Interleukin-6-Stimulated Sonic Hedgehog Signaling in Human Acute Myeloid Leukemia
Figure 5
Effect of cyclopamine and resveratrol on Gli-1 nuclear translocation in HL-60 cells with or without IL-6 treatment. (a) Expression of nucleic and cytosolic Gli-1 using a Western blot analysis. The relative values were normalized to the internal control, were calculated from three independent experiments, and were expressed as mean ± SE. (b) Immunofluorescence staining of Gli-1. These cells were double stained with PE-conjugated anti-Gli-1 antibody (red) and Hoechst 33342 fluorescence dye (blue, nucleus). as compared to the control group. as compared to the IL-6 alone group.
