Destruxin B Isolated from Entomopathogenic Fungus Metarhizium anisopliae Induces Apoptosis via a Bcl-2 Family-Dependent Mitochondrial Pathway in Human Nonsmall Cell Lung Cancer Cells
Figure 5
The effects of destruxin B on H1299 cells. (a) Antiproliferative effect. H1299 cells were untreated or treated with 1, 5, 10, 20, and 30 μM of destruxin B for 48 h. The cell number was measured. (b) Activation of caspases. H1299 cells were treated with 20 μM destruxin B for 48 h. The cells were then lyzed and incubated with the fluorescein-conjugated substrates of caspase-2, -3, and -9 and subjected to the microplate reader to detect caspase activity. versus control; versus control; versus control. (c) H1299 cells were treated with 0, 10, or 20 μM destruxin B for 12 and 24 h. The cells were collected, lyzed, and subjected to western blot with antibodies against PUMA, Mcl-1, and β-actin, respectively.
