Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 2

Research Article

In Vitro Evaluations of Cytotoxicity of Eight Antidiabetic Medicinal Plants and Their Effect on GLUT4 Translocation

Figure 2

Effect of plant extracts on GLUT4 translocation. L6-GLUT4myc cells were seeded in 24-well plate (100,000 cells/well) and exposed to Allium sativum (a), Allium cepa (b), Trigonella foenum-graecum (c), Olea europaea (d), Nigella Sativa (e), Urtica pilulifera (f), Atriplex halimus (G), and Cinnamomum zeylanicum (h) for 24 h. Serum depleted cells were treated without or with 100 nM insulin for 20 min at 37°C and surface myc-tagged GLUT4 density was quantified using the antibody coupled colorimetric assay. Shown are the means ± S.E relative to basal nontreated cells from three independent experiments (each has three triplicates).
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