Cytotoxic Activity of Kenaf Seed Oils from Supercritical Carbon Dioxide Fluid Extraction towards Human Colorectal Cancer (HT29) Cell Lines
Morphological study of HT29 cell lines treated with various concentration of KSO-SFE after 72 h. (a) 0 μg/mL (control); (b) 200 μg/mL; (c) 1000 μg/mL viewed under a fluorescence microscope (0x magnification). (d) Cell blebbing (100x magnification). Viable cells were round stained green by acridine orange, while dead cells were stained red by propidium iodide. The arrows in (d) showed blebbed cell that indicate the apoptosis characteristics. KSO-SFE: kenaf seed oil extracted by supercritical fluid extraction.