Naringenin Inhibits Adipogenesis and Reduces Insulin Sensitivity and Adiponectin Expression in Adipocytes
Figure 1
Naringenin blocks differentiation of murine 3T3-L1 preadipocytes. Preadipocytes were induced to differentiate using the typical MDI induction cocktail. Immediately following induction ((a) and (b)) or at the indicated time point post-MDI (c), cells were treated with DMSO vehicle (V), the indicated the dose of naringenin ((a) and (b)), or 25 μg/mL naringenin (c). The cells were retreated with the indicated dose of naringenin following each media change every 48–72 hours as indicated in the methods. The control cells (C) received no treatment. Each panel is representative of 3–6 independently performed experiments. Oil Red O staining of neutral lipid content was performed 7 days post-MDI (a). Fifty to one hundred fifty micrograms of protein from whole cell extracts, harvested at four (b) or seven (c) days post-MDI were subjected to western blot analysis. Band intensities were quantified, normalized against the loading control, MAPK, and presented as a bar graph next to each immunoblot. Similar dose-dependent patterns of adipocyte marker protein expression were observed when cells were harvested at either 4 or 7 days post-MDI, and panel (b) contains a representative subset of these blots.
