Naringenin Inhibits Adipogenesis and Reduces Insulin Sensitivity and Adiponectin Expression in Adipocytes
Figure 4
Naringenin inhibits adiponectin expression in mature 3T3-L1 and subcutaneous human adipocytes. Mature 3T3-L1 adipocytes cultured in 6 well plates were treated with 50 μg/mL naringenin (Nar) or DMSO vehicle control (C) for the indicated length of time prior to the preparation of whole cell extracts (a). For each time point, data from two individual wells are shown. Subcutaneous human adipocytes cultured in 12 well plates were treated with 25 μg/mL naringenin (Nar) for 72 hours prior to harvesting (b). The control cells (C) received no treatment. For the 48- and 72-hour treatments, cells were treated daily, and the medium was replaced 24 hours prior to harvesting the whole cell extracts. Fifty to one hundred fifty micrograms of protein from whole cell extracts were separated by SDS-PAGE, transferred to nitrocellulose, and used for western blot analysis. The anti-mouse adiponectin antibody was used to probe the samples in (a), whereas the anti-mouse/human adiponectin antibody was use to probe the samples in (b). Band intensities were quantified, normalized against the loading control, MAPK, and presented as fold change relative to 24 h control (a) or control (b) in a bar graph beneath each immunoblot. Values for duplicate samples were averaged, and data are presented as mean ± SEM.
