Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication
Figure 1
Effect of PGSM on HCV protein expression and RNA replication in HCV replicon cells. Huh7 cells harboring HCV replicon were treated with different concentrations of PGSM for 3 days. 1% DMSO was used as a control (vehicle). (a) Total cell lysates were immunoblotted with an anti-HCV NS5A antibody and anti-actin antibody, respectively. (b) Total RNAs were extracted from cells at 72 h after PGSM treatment, and intracellular HCV RNAs were quantified by qPCR. Relative HCV RNA levels were normalized by cellular GAPDH mRNA. Cell viability was assessed by the MTT assay.
