Huang Qi Jian Zhong Pellet Attenuates TNBS-Induced Colitis in Rats via Mechanisms Involving Improvement of Energy Metabolism

Table 2

The number of animals in different experimental groups for various parameters.

	Sham 15 d	Sham 15 d + HQJZ	TNBS 1 d	TNBS 15 d	TNBS 15 d + HQJZ	Total
Colonic microcirculation	6	6	6	6	6	30
Colonic blood flow, macroscopical and microscopical evaluation, expression of CD11b and, cytokines	8	8	8	8	8	40
Immunohistochemistry and immnofluorescence staining	(3)	(3)	(3)	(3)	(3)
Western blot analysis	(5)	(5)	(5)	(5)	(5)
Total	14	14	14	14	14	70

The animals were separated as two batches in the present study. Only one batch of animals (30 rats) was used to observe colonic microcirculation. And the other parameters were analyzed using the second batch of animals (40 rats). The same animals were used for detection of colonic blood flow, macroscopical and microscopical evaluation, and expression of CD11b and cytokines. Brackets: the tissues for immunohistochemistry, immnofluorescence staining, and Western blotting analysis were removed from the second batch of animals (40 rats). Sham 15 d: the rats received physiological saline by enema and 24 h thereafter physiological saline by: gavage for 14 days; Sham 15 d + HQJZ: the rats received physiological saline by enema and 24 h thereafter HQJZ at 2 g/kg by gavage for 14 days; TNBS 1 d, the rats received TNBS by enema, and were sacrificed after 24 h; TNBS 15 d: the rats received TNBS by enema and 24 h thereafter: physiological saline by gavage for 14 days; TNBS 15 d + HQJZ: the rats received TNBS by enema and 24 h thereafter: HQJZ at 2 g/kg everyday by gavage for 14 days.