Antiteratogenic Effects of -Carotene in Cultured Mouse Embryos Exposed to Nicotine
Figure 8
Representative images of apoptotic embryos exposed to nicotine and β-carotene by Nile blue staining. Nile blue staining was performed to observe apoptotic nuclei and dead cells which stained dark blue. Normal control embryos (a). Embryos treated with 1 mM nicotine exhibit increased levels of apoptosis (b). Embryos treated with 1 mM nicotine plus β-carotene [1 × 10−7μM (c) and 5 × 10−7μM (d)] appear similar to the control group.