Resveratrol Impedes the Stemness, Epithelial-Mesenchymal Transition, and Metabolic Reprogramming of Cancer Stem Cells in Nasopharyngeal Carcinoma through p53 Activation
Figure 5
Resveratrol impeded EMT through p53 activation in CSCs. (a) Expressions of p53 and phosphorylated p53 (Ser15) were lower in TW01 CSC than those in TW01 parental cells. Resveratrol could increase expression of p53 and phosphorylated p53 expression both in CSC and parental cells. (b) Resveratrol (50 μM) reactivated p53, indicated by the translocation of phosphorylated p53 (Ser15) from cytoplasm to the nucleus (detected with DAPI staining), and repressed the EMT phenotype in TW01 CSCs. TW01 parental cells remained epithelial type after resveratrol treatment. Scale bars indicate 40 μm. (c) Western blot indicated the p53 expression of TW01 CSCs with 50 μM resveratrol treatment (RV) or p53 overexpression . (d) Western blot confirmed the p53 knockdown efficiency in TW01 CSCs. shp53#2 with the best knockdown efficiency was chose for further experiment.
