Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation

Figure 3

Influence of ellagic acid on protein kinase C (PKC) activation and platelet aggregation in activated platelets. Washed platelets were preincubated with 50 and 80 μM ellagic acid or 0.5% DMSO, followed by the addition of 1 μg/mL collagen or 150 nM PDBu to trigger ((a)-(b)) PKC activation (p47 phosphorylation) and (c) platelet aggregation, as described in the Materials and Methods. The data are presented as the means ± S.E.M. (𝑛=4). **𝑃<0.01, compared with the control (resting) group; ^#𝑃<0.05 and ^##𝑃<0.01, compared with the collagen-treated group. The profiles (c) show representative examples of 3 similar experiments.

(a)

(b)

(c)