Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation
The effects of ellagic acid in regulating hydroxyl radical (OH●) formation and p38 MAPK and Akt activation in platelets. (a) For the electron spin resonance (ESR) study, washed platelets were (A) incubated with 0.5% DMSO only (resting group) or preincubated with (B) 0.5% DMSO, (C) 50 μM ellagic acid, and (D) 80 μM ellagic acid, followed by the addition of 1 μg/mL collagen to trigger OH● formation. For other experiments, washed platelets were preincubated with 50 and 80 μM ellagic acid or 0.5% DMSO, followed by the addition of 1 mM hydrogen peroxide to trigger platelet activation. The cells were collected, and subcellular extracts were analyzed for (b) p38 MAPK and (c) Akt phosphorylation. All profiles are representative examples of 4 similar experiments.
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