Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 5

Research Article

Novel Bioactivity of Ellagic Acid in Inhibiting Human Platelet Activation

Figure 5

The effects of ellagic acid in regulating hydroxyl radical (OH) formation and p38 MAPK and Akt activation in platelets. (a) For the electron spin resonance (ESR) study, washed platelets were (A) incubated with 0.5% DMSO only (resting group) or preincubated with (B) 0.5% DMSO, (C) 50 μM ellagic acid, and (D) 80 μM ellagic acid, followed by the addition of 1 μg/mL collagen to trigger OH formation. For other experiments, washed platelets were preincubated with 50 and 80 μM ellagic acid or 0.5% DMSO, followed by the addition of 1 mM hydrogen peroxide to trigger platelet activation. The cells were collected, and subcellular extracts were analyzed for (b) p38 MAPK and (c) Akt phosphorylation. All profiles are representative examples of 4 similar experiments.

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