Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Tab 2 / Research Article
Validation of the Antidiabetic and Hypolipidemic Effects of Hawthorn by Assessment of Gluconeogenesis and Lipogenesis Related Genes and AMP-Activated Protein Kinase Phosphorylation Table 2 Primers used in this study.
Gene Accession number Forward primer and reverse primer PCR product (bp) Annealing temperature (°C) White adipose tissue PPARγ NM_013124 F: CATGCTTGTGAAGGATGCAAG 190 55 Skeletal muscle Glut4 M25482 F: ACTGGCGCTTTCACTGAACT 106 56 Liver PPARα NM_011144 F: ACCTCTGTTCATGTCAGACC 352 55 apoA-I NM_009692.3 F: ACATATATAGACCAGGGAAGAA 246 50.5 apoC-III NM_023114.3 F: CAGTTTTATCCCTAGAAGCA 349 47 FAS NM_007988 F: TGGAAAGATAACTGGGTGAC 240 50 PEPCK NM_011044.2 F:CTACAACTTCGGCAAATACC 330 52 SREBP1c NM_011480 F: GGCTGTTGTCTACCATAAGC 219 50 SREBP2 AF289715.2 F: ATATCATTGAAAAGCGCTAC 256 47 GAPDH NM_031144 F: TGTGTCCGTCGTGGATCTGA 99 55
