Rosmarinic Acid and Its Methyl Ester as Antimicrobial Components of the Hydromethanolic Extract of Hyptis atrorubens Poit. (Lamiaceae)

Table 3

Results of quantitative analysis of the four compounds by HPLC and linearity of calibration.

Compounds	Retention time; (min)	Linear range; (mg/mL)	y = ax + b; (linear model)	Correlation coefficient; (𝑅^2)	Percent in total extract; (%)
RA	20.67±0.45	0.125–1.000	5898776=(2×10^−7)𝑥+243823	0.9996	5.60
MR	38.77±0.52	0.030–1.000	705509=(2×10^−7)𝑥+38893	0.9995	0.03
IQ	15.38±0.96	0.006–0.500	103118=(2×10^−7)𝑥−31067	0.9999	0.08
HS	17.88±0.85	0.006–0.500	218757=(2×10^−7)𝑥−57439	0.9995	0.06

𝑦: peak area of compound in total extract, 𝑥: concentration of compound in total extract.