Table 2: Fold changes of differential proteins in aqueous- and methanolic-treated PC-3 cells. Symbols “+” indicate upregulation and “−” indicate downregulation. PA: P. amarus; PN: P. niruri; PU: P. urinaria and PW: P. watsonii.

No.UniProtKB/Swiss-Prot 
(accession number)
ProteinPhyllanthus
Aqueous extractMethanolic extract
PAPNPUPWPAPNPUPW

ICell adhesion, migration, invasion, and metastasis and angiogenesis

1P98172Ephrin-B1−1.53−1.45−1.21−1.41−2.14−2.21−2.01−2.31
2P05787Keratin, type II cytoskeletal 81.451.321.381.211.861.741.921.62
3P63261Actin, cytoplasmic 2−1.43−1.53−1.21−1.53−1.74−1.79−1.72−1.53
4Q8NDI1EH domain-binding protein 1−1.32−1.32−1.21−1.13−1.42−1.32−1.43−1.43
5P04792Heat shock protein beta-1−1.43−1.32−1.54−1.54−1.42−1.43−1.32−1.25
6P35527Keratin, type I cytoskeletal 91.381.321.531.421.641.341.351.37
7Q9BYR8Keratin-associated protein 3-11.531.321.471.641.471.531.421.46
8P08670Vimentin−1.43−1.32−1.32−1.11−1.25−1.43−1.32−1.42
9Q9NY65Tubulin alpha-8 chain−1.53−1.43−1.48−1.35−1.40−1.43−1.42−1.73
10Q9Y316Protein MEMO1−1.43−1.42−1.24−1.32−1.63−1.32−1.42−1.43

IIProliferation, cell cycle, and apoptosis

11Q92466DNA damage-binding protein 2−1.42−1.32−1.43−1.41−1.42−1.37−1.43−1.32
12Q7RTU1Transcription factor 23−1.39−1.33−1.42−1.43−1.53−1.63−1.58−1.42
13Q9UQ80Proliferation-associated protein 2G4−1.37−1.57−1.42−1.47−1.63−1.45−1.53−1.43
14P62993Growth factor receptor-bound protein 2−1.42−1.52−1.43−1.32−1.42−1.42−1.43−1.64
15O60565Gremlin-1−1.43−1.43−1.33−1.43−1.56−1.43−1.54−1.44
16P2734814-3-3 protein theta−1.32−1.43−1.53−1.42−1.42−1.21−1.32−1.42
17P6198114-3-3 protein gamma−1.32−1.32−1.43−1.35−1.32−1.42−1.22−1.32
18P04083Annexin A1−1.52−1.54−1.42−1.43−1.64−1.43−1.43−1.43
19Q96LY2Coiled-coil domain-containing protein 74B−1.47−1.34−1.47−1.46−1.33−1.33−1.36−1.57
20P31943Heterogeneous nuclear ribonucleoprotein H−1.47−1.33−1.35−1.21−1.44−1.56−1.43−1.53
21P09211Glutathione S-transferase P−1.96−1.84−1.77−2.05−1.89−1.87−2.01−1.97
22P41221Protein Wnt-5a−1.75−1.87−1.87−1.92−1.74−1.84−1.85−2.01
23Q9H0R3Transmembrane protein 222−1.57−1.52−1.57−1.42−1.47−1.62−1.57−1.46
24Q5MJ09Sperm protein associated with the nucleus on the X chromosome N3−1.45−1.57−1.57−1.57−1.34−1.45−1.32−1.46
25P46063ATP-dependent DNA helicase Q1−1.57−1.57−1.36−1.46−1.56−1.47−1.46−1.57
26P09382Galectin-1−1.25−1.46−1.57−1.47−1.46−1.33−1.46−1.46
27P04792Heat shock protein beta-1−1.37−1.47−1.47−1.46−1.56−1.46−1.34−1.47
28P78417Glutathione transferase omega-1−1.46−1.46−1.47−1.47−1.57−1.54−1.68−1.57
29Q06830Peroxiredoxin-1−1.57−1.42−1.29−1.33−1.48−1.62−1.54−1.67
30P30048Thioredoxin-dependent peroxide reductase, mitochondrial−1.45−1.52−1.12−1.64−1.58−1.44−1.57−1.57
31Q9Y230RuvB-like 2−1.46−1.47−1.38−1.32−1.75−1.57−1.64−1.57
32P50453Serpin B9−1.46−1.50−1.36−1.38−1.58−1.67−1.76−1.63
33Q8ND25E3 ubiquitin-protein ligase ZNRF1−1.56−1.43−1.47−1.47−1.58−1.67−1.82−1.54
34Q6PRD1Probable G-protein-coupled receptor 179−1.45−1.43−1.47−1.56−1.36−1.37−1.54−1.58
35O43521Bcl-2-like protein 11−1.66−1.52−1.42−1.56−1.57−1.73−1.651.56
36P56703Proto-oncogene Wnt-3 precursor−2.18−1.92−2.06−2.34−1.79−1.971.83−2.11
37Q8N4Z0Putative Ras-related protein Rab-42−2.11−2.03−1.79−1.98−1.96−2.1−2.15−1.94
38P01112GTPase HRas precursor−2.12−1.99−1.83−1.74−1.85−2.03−2.05−1.92

IIIGlycogenesis and glycolysis

39Q969E3Urocortin-3−1.37−1.42−1.37−1.46−1.65−1.731.67−1.47
40P00558Phosphoglycerate kinase 1−2.01−2.12−2.22−1.93−1.56−1.37−1.57−1.36
41P06733Alpha-enolase−1.42−1.38−1.37−1.36−1.58−1.57−1.65−1.57
42P04406Glyceraldehyde-3-phosphate dehydrogenase−1.92−1.88−1.92−1.83−1.78−1.36−1.36−1.56
43P04075Fructose-bisphosphate aldolase A−1.63−1.42−1.39−1.36−1.48−1.53−1.52−1.57
43P60174Triosephospate isomerase−1.47−1.46−1.47−1.76−1.48−1.53−1.49−1.49
44Q9NPG2Neuroglobin−1.47−1.47−1.58−1.47−1.56−1.63−1.74−1.74

IVProtein synthesis and energy metabolism

45Q4U2R639S ribosomal protein L51−1.37−1.47−1.36−1.53−1.64−1.74−1.65−1.67
46Q93088Betaine-homocysteine S-methyltransferase 1 −1.46−1.47−1.46−1.48−1.67−1.48−1.58−1.57
47P50583Bis(5′-nucleosyl)-tetraphosphatase [asymmetrical]−1.45−1.57−1.30−1.42−1.39−1.47−1.67−1.87
48Q96A11Galactose-3-O-sulfotransferase 3−1.69−1.74−1.67−1.85−1.93−1.74−1.82−1.89
49Q9Y274Type 2 lactosamine alpha-2,3-sialyltransferase−1.28−1.52−1.58−1.44−1.68−1.64−1.58−1.57
50O43852Calumenin−1.53−1.47−1.47−1.42−1.57−1.65−1.56−1.53
51P13667Protein disulfide-isomerase A4−1.47−1.41−1.44−1.45−1.58−1.64−1.45−1.57
52P27797Calreticulin−1.54−1.32−1.47−1.48−1.45−1.53−1.57−1.64
53P07900Heat shock protein HSP 90-alpha−1.64−1.32−1.34−1.43−1.55−1.42−1.32−1.53
54P12235ADP/ATP translocase 1−1.46−1.38−1.45−1.35−1.58−1.68−1.67−1.47
55P522096-phosphogluconate dehydrogenase, decarboxylating−1.46−1.49−1.54−1.45−1.57−1.52−1.48−1.56
56Q9Y4785′-AMP-activated protein kinase subunit beta-1−1.38−1.46−1.47−1.47−1.67−1.75−1.47−1.57
57P1102178 kDa glucose-regulated protein−1.46−1.46−1.47−1.57−1.57−1.54−1.57−1.84
58Q9UI09NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12−1.42−1.36−1.33−1.33−1.57−1.73−1.57−1.46
59P30101Protein disulfide-isomerase A3−1.20−1.47−1.37−1.21−1.57−1.63−1.56−1.57
60P49411Elongation factor Tu, mitochondrial−1.48−1.45−1.57−1.33−1.38−1.54−1.68−1.47
61Q5TGZ0Mitochondrial inner membrane organizing system protein 1−1.47−1.46−1.47−1.49−1.57−1.47−1.67−1.65
62P19404NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial−1.21−1.32−1.33−1.43−1.57−1.57−1.57−1.74
63Q9H0F7ADP-ribosylation factor-like protein 6−1.76−1.50−1.57−1.54−1.56−1.54−1.49−1.70
64O75436Vacuolar protein sorting-associated protein 26A−1.46−1.54−1.63−1.76−1.47−1.38−1.57−1.57
65Q9NXV2BTB/POZ domain-containing protein KCTD5−1.47−1.47−1.33−1.50−1.58−1.65−1.67−1.66
66O00429Dynamin-1-like protein−1.47−1.63−1.47−1.57−1.56−1.57−1.67−1.73
67P40261Nicotinamide N-methyltransferase−1.29−1.22−1.34−1.28−1.45−1.48−1.83−1.42
68P49720Proteasome subunit beta type-3−1.53−1.22−1.34−1.47−1.58−1.53−1.67−1.57
69Q6IQ16Speckle-type POZ protein-like−1.42−1.67−1.47−1.47−1.63−1.64−1.68−1.47
70P21796Voltage-dependent anion-selective channel protein 11.571.631.521.791.671.841.771.76
71Q9Y3D8Adenylate kinase isoenzyme 6−1.51−1.33−1.43−1.49−1.46−1.64−1.74−1.76
72Q14152Eukaryotic translation initiation factor 3 subunit 12−1.40−1.37−1.39−1.49−1.53−1.67−1.63−1.53