Gallic Acid Induces a Reactive Oxygen Species-Provoked c-Jun NH2-Terminal Kinase-Dependent Apoptosis in Lung Fibroblasts
Figure 1
Involvement of JNK activation in gallic acid-mediated apoptosis. (a) Time effect. MLFs were treated with 50 g/mL gallic acid for 0.5, 1, and 2. (b) Dose effect. MLFs were treated with 0, 10, 30, and 50 g/mL gallic acid for 1 h. After incubation, the activation of p-Akt, p-ERK, p-JNK, and p-p38MAPK was detected by Western blot analysis. -Actin was used as an internal loading control. (c) MLFs were pretreated in the presence of the specific inhibitors of Akt, ERK, and JNK (LY294002, U0126, and SP600125, respectively) for 1 h and then incubated with 50 g/mL gallic acid for 24 h. The apoptotic cells were determined by TUNEL assay. Data were expressed as the mean SD from 3 independent experiments.
